{"id":80,"date":"2017-11-15T20:42:12","date_gmt":"2017-11-16T01:42:12","guid":{"rendered":"http:\/\/sites.williams.edu\/ion\/?p=80"},"modified":"2018-03-24T12:19:47","modified_gmt":"2018-03-24T16:19:47","slug":"improved-fluorescence-collection","status":"publish","type":"post","link":"https:\/\/sites.williams.edu\/ion\/uncategorized\/improved-fluorescence-collection\/","title":{"rendered":"Improved Fluorescence Collection"},"content":{"rendered":"<p>Our lab uses a home-built high NA lens based on a design by Curtis Volin from GTRI.\u00a0 Our original lens was performing poorly; it collected lots of light, but did not focus it terribly tightly.\u00a0 So, using the freeware raytracer Beam4 we have tweaked another one of Volin&#8217;s designs and built a new lens:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-84\" src=\"https:\/\/sites.williams.edu\/ion\/files\/2017\/12\/Lens2_optimized-300x232.png\" alt=\"\" width=\"300\" height=\"232\" srcset=\"https:\/\/sites.williams.edu\/ion\/files\/2017\/12\/Lens2_optimized-300x232.png 300w, https:\/\/sites.williams.edu\/ion\/files\/2017\/12\/Lens2_optimized-768x593.png 768w, https:\/\/sites.williams.edu\/ion\/files\/2017\/12\/Lens2_optimized-1024x791.png 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>This design uses four Thorlabs stock lenses and achieves a numerical aperture of roughly 0.42.\u00a0 With the rebuilt lens we&#8217;re now collecting approximately 15% more light and focusing it into a spot about half as large.\u00a0 Still not diffraction limited, but progress!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our lab uses a home-built high NA lens based on a design by Curtis Volin from GTRI.\u00a0 Our original lens was performing poorly; it collected lots of light, but did not focus it terribly tightly.\u00a0 So, using the freeware raytracer &hellip; <a href=\"https:\/\/sites.williams.edu\/ion\/uncategorized\/improved-fluorescence-collection\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":996,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-80","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/posts\/80","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/users\/996"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/comments?post=80"}],"version-history":[{"count":2,"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/posts\/80\/revisions"}],"predecessor-version":[{"id":312,"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/posts\/80\/revisions\/312"}],"wp:attachment":[{"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/media?parent=80"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/categories?post=80"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.williams.edu\/ion\/wp-json\/wp\/v2\/tags?post=80"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}