Improved Fluorescence Collection

Our lab uses a home-built high NA lens based on a design by Curtis Volin from GTRI.  Our original lens was performing poorly; it collected lots of light, but did not focus it terribly tightly.  So, using the freeware raytracer Beam4 we have tweaked another one of Volin’s designs and built a new lens:

This design uses four Thorlabs stock lenses and achieves a numerical aperture of roughly 0.42.  With the rebuilt lens we’re now collecting approximately 15% more light and focusing it into a spot about half as large.  Still not diffraction limited, but progress!

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